誘電泳動速度計測による熱処理酵母の代謝評価―低温加熱における処理時間の影響―

カテゴリ: 論文誌(論文単位)

グループ名: 【E】センサ・マイクロマシン部門

発行日: 2016/12/01

タイトル(英語): Metabolic Evaluation of Heat-Treated Yeast by Dielectrophoretic Velocimetry―Influence of Treatment Time at Low Temperature―

著者名: 石田　高広（首都大学東京），白井　直機（首都大学東京），内田　諭（首都大学東京），杤久保　文嘉（首都大学東京）

著者名(英語): Takahiro Ishida (Tokyo Metropolitan University), Naoki Shirai (Tokyo Metropolitan University), Satoshi Uchida (Tokyo Metropolitan University), Fumiyoshi Tochikubo (Tokyo Metropolitan University)

キーワード: 誘電泳動，速度計測，加熱処理，酵母，マイクロセル，代謝評価　　dielectrophoresis，velocimetry，heat treatment，yeast，micro cell，metabolic evaluation

要約(英語): Constant monitoring and detailed examination of microbial metabolism have been required for advanced quality control in bioindustry. Although microbial evaluation using dielectrophoresis (DEP) should be an attractive scheme, the relationship with metabolism has not been understood well. In this paper, the membrane states and metabolic change of injured Saccharomyces cerevisiae (S. cerevisiae) with heat treatment were investigated using DEP velocimetry in a micro cell. As a result, the treatment time at low temperature and afterheat stress sensitively influenced DEP velocity of S. cerevisiae. The fluorescent observation of stained S. cerevisiae indicated DEP velocity has a correlation with the transmembrane state and growth activity. These results suggest that DEP velocimetry would be available for rapid and simple metabolic evaluation.

本誌: 電気学会論文誌E（センサ・マイクロマシン部門誌） Vol.136 No.12 （2016）

本誌掲載ページ: 505-510 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejsmas/136/12/136_505/_article/-char/ja/