Microchanneled Calorimetric Concentration Sensor for Picoliter Liquid Samples of Cytochrome c

カテゴリ: 論文誌(論文単位)

グループ名: 【E】センサ・マイクロマシン部門

発行日: 2017/01/01

タイトル(英語): Microchanneled Calorimetric Concentration Sensor for Picoliter Liquid Samples of Cytochrome c

著者名: Masaya Toda (Department of Mechanical Systems Engineering, Graduate School of Engineering, Tohoku University), Ning Xia (Department of Mechanical Systems Engineering, Graduate School of Engineering, Tohoku University), Naoki Inomata (Department of Mechani

著者名(英語): Masaya Toda (Department of Mechanical Systems Engineering, Graduate School of Engineering, Tohoku University), Ning Xia (Department of Mechanical Systems Engineering, Graduate School of Engineering, Tohoku University), Naoki Inomata (Department of Mechanical Systems Engineering, Graduate School of Engineering, Tohoku University), Takahito Ono (Department of Mechanical Systems Engineering, Graduate School of Engineering, Tohoku University)

キーワード: micro-calorimeter，protein，bio-sensing

要約(英語): A calorimetric determination of protein concentration for a picoliter (pL) liquid sample is proposed using a microfluidic calorimeter on a thermally isolated membrane. The concentration measurement of cytochrome c in a pL solution based on an AC calorimeter by the absorbance of a 532 nm diode laser is demonstrated to monitor the temperature change of the microchannel. The resolution of the concentration is defined by measuring the temperature change against various concentrations. In the case of the fabricated micro-calorimeter, the heat resistance of the isolated membrane is obtained as 1579 K/W. The composed heat resistance, including the support membrane and the solution in the channel, is obtained as 118 K/W. Although the heat loss is extensive, the resolution is calculated as 3.3 mM as a concentration sensor with 1.5 mol/K sensitivity. The calorimetric device has more potential to be used for detection of extremely small concentrations in liquid by optimizing the thermal isolation with a higher concentration resolution.

本誌: 電気学会論文誌E（センサ・マイクロマシン部門誌） Vol.137 No.1 （2017） 特集：センサ・マイクロマシン英文特集号

本誌掲載ページ: 28-31 p

原稿種別: 論文／英語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejsmas/137/1/137_28/_article/-char/ja/