Development of an ex vivo Brain Lactic Acid Monitoring System

カテゴリ: 論文誌(論文単位)

グループ名: 【E】センサ・マイクロマシン部門

発行日: 2017/01/01

タイトル(英語): Development of an ex vivo Brain Lactic Acid Monitoring System

著者名: Shuya Negishi (Department of Electronics and Bioinformatics, School of Science and Technology, Meiji University), Shoto Kamada (Department of Electronics and Bioinformatics, School of Science and Technology, Meiji University), Riichi Kajiwara (Department

著者名(英語): Shuya Negishi (Department of Electronics and Bioinformatics, School of Science and Technology, Meiji University), Shoto Kamada (Department of Electronics and Bioinformatics, School of Science and Technology, Meiji University), Riichi Kajiwara (Department of Electronics and Bioinformatics, School of Science and Technology, Meiji University), Yuji Kaneko (Department of Electronic & Physical Systems Major in Nano-Science & Nano-engineering, Waseda University), Dong Hyun Yoon (Department of Electronic & Physical Systems Major in Nano-Science & Nano-engineering, Waseda University), Tetsushi Sekiguchi (Department of Electronic & Physical Systems Major in Nano-Science & Nano-engineering, Waseda University), Shuichi Shoji (Department of Electronic & Physical Systems Major in Nano-Science & Nano-engineering, Waseda University), Hiroyuki Kudo (Department of Electronics and Bioinformatics, School of Science and Technology, Meiji University)

キーワード: Flow injection，lactic acid，brain，ex vivo biomonitoring，electrochemical，real-time

要約(英語): An ex vivo brain lactic acid (LA) monitoring system for investigating brain activity at the organ level was constructed and tested in continuous LA monitoring. Our system consists of a micro-fluidic dual-analyte (LA and glucose) biosensor and a special brain chamber that is suitable for organ level experiments. The biosensor utilizes the redox reactions of osmium-wired horseradish peroxidase (Os-HRP) to detect hydrogen peroxide produced by enzyme reactions. The sensor had sufficient sensitivity (LA: ～ 0.01 mM, glucose: ～ 0.01 mM) and selectivity for assessment of extracellular LA. The ex vivo monitoring of brain LA was performed using the proposed system. As a result, the change in extracellular LA was successfully monitored continuously, and the change in the LA level was considered the result of metabolic activity of the sample. Our system is expected to be useful not only for ex vivo brain monitoring, but also for monitoring other organs.

本誌: 電気学会論文誌E（センサ・マイクロマシン部門誌） Vol.137 No.1 （2017） 特集：センサ・マイクロマシン英文特集号

本誌掲載ページ: 38-43 p

原稿種別: 論文／英語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejsmas/137/1/137_38/_article/-char/ja/