低濃度乳酸モニタリングのための酵素プリンティングによる酵素電極センサ

カテゴリ: 論文誌(論文単位)

グループ名: 【E】センサ・マイクロマシン部門

発行日: 2018/06/01

タイトル(英語): Enzyme Printed Enzyme Electrode Sensor for Low Concentration Lactate Monitoring

著者名: 鶴岡　典子（東北大学），松永　忠雄（東北大学），井上(安田)　久美（東北大学），末永　智一（東北大学），芳賀　洋一（東北大学）

著者名(英語): Noriko Tsuruoka (Tohoku University), Tadao Matsunaga (Tohoku University), Kumi Y. Inoue (Tohoku University), Tomokazu Matsue (Tohoku University), Yoichi Haga (Tohoku University)

キーワード: 酵素電極，Os-HRP，ラクテートオキシダーゼ，インクジェットプリンタ，塗布針式塗布装置　　enzyme electrode，Os-HRP，lactate oxidase，inkjet printer，pasting needle type printer

要約(英語): In this research, enzyme electrode sensor for measuring low concentration lactate was fabricated. In our previous work, microperfusion needle to measure concentration of lactate in subepidermal tissue have been developed. Using this microperfusion needle, lactate concentration of perfusate was about one percent of lactate concentration of blood. In order to measure this low concentration of lactate, enzyme electrode sensor using printed enzyme was fabricated. Au electrodes were patterned on the glass substrate. Ag nanometal ink was printed on reference electrode. Os-HRP polymer was printed by pasting needle type printer and lactate oxidase was printed by inkjet printer on working electrode. Addition agents which do not inactivate enzymes as wetting agent and viscosity modifier were selected. A flow channel was pasted on fabricated electrodes to measure concentration changes continuously. Fabricated sensor had high sensitivity and could measure low concentration lactate (0.01-0.25 mM). In addition, fabricated sensor was not affected by interfering substances.

本誌: 電気学会論文誌E（センサ・マイクロマシン部門誌） Vol.138 No.6 （2018）

本誌掲載ページ: 231-240 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejsmas/138/6/138_231/_article/-char/ja/