微小孔アレイを用いたミクロンサイズ細胞外ベシクルの生成分離

カテゴリ: 論文誌(論文単位)

グループ名: 【E】センサ・マイクロマシン部門

発行日: 2019/07/01

タイトル(英語): Production and Separation of Micrometer-size Extracellular Vesicles Using Microhole Arrays

著者名: 櫻田　一歩（九州工業大学 大学院生命体工学研究科），安田　隆（九州工業大学 大学院生命体工学研究科）

著者名(英語): Kazuho Sakurada (Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology), Takashi Yasuda (Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology)

キーワード: 細胞外ベシクル，リンパ球様細胞，微小孔，窒化ケイ素，細胞膜アンカー　　extracellular vesicle，lymphocyte，microhole，silicon nitride，cell membrane anchor

要約(英語): We developed a technique for producing and separating micrometer-size vesicles from human lymphocytes using microholes. Using cell-anchoring molecules, lymphocytes were immobilized on a microhole array formed in a silicon nitride (SiN) membrane. Then, the cells were chemically stimulated with sodium butyrate, and the vesicle production was induced near the microholes with the assistance of the cell-anchoring molecules. This resulted in the vesicle production inside the microholes, and the vesicles finally passed through the microholes. We tested microholes having 3 different diameters of 2, 4, and 7.5 μm, and counted the number of fluorescently-stained vesicles and cells which passed through microholes of each diameter. The number of vesicles increased with increasing diameter of microholes, but the number of invading cells also increased. In case of microholes 4 μm in diameter, a relatively large number of vesicles were obtained, and the cell invasion rate was below 1%, which was the best result in the present experiment. Also, we succeeded in drastically increasing the number of vesicles in case of microholes 2 μm in diameter by preventing vesicles from adhering to the back side of a SiN membrane.

本誌: 電気学会論文誌E（センサ・マイクロマシン部門誌） Vol.139 No.7 （2019） 特集：第35回「センサ・マイクロマシンと応用システム」シンポジウム受賞論文

本誌掲載ページ: 195-200 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejsmas/139/7/139_195/_article/-char/ja/