内部光電効果を用いた電気化学アレイセンサによる酸化体メディエータの還元電流測定の研究

カテゴリ: 論文誌(論文単位)

グループ名: 【E】センサ・マイクロマシン部門

発行日: 2019/12/01

タイトル(英語): Study on Measurement of Reduction Current of Oxidant Mediator by Electrochemical Array Sensor Using Internal Photoelectric Effect

著者名: 小杉　恵太（埼玉大学），内田　秀和（埼玉大学）

著者名(英語): Keita Kosugi (Saitama University), Hidekazu Uchida (Saitama University)

キーワード: 酸化還元酵素，内部光電効果，LAAS，電気化学測定法，メディエータ，ショットキー接合　　oxidoreductase，internal photoelectric effect，LAAS，electrochemical measurement method，mediator，schottky junction

要約(英語): Enzymes which have potential to detect specific chemical substances are important molecules in drug discovery, food and medical diagnosis. The most powerful method for evaluation of enzyme activity is fluorescence technique. However, there is a problem that the fluorescence methods such as Fluorescence Resonance Energy Transfer (FRET) need development of fluorescence molecules. In our previous study, we have developed the arrayed electrochemical sensors for oxidation current measurement of redox mediator in the reaction of oxidoreductase which oxidizes mediator with catalysis. In this study, we fabricated the electrochemical array sensor to measure reduction current of oxidized mediator. The sensor is based on an internal photoelectric effect. The concentration dependency of observed current on single sensor element shows monotonic dependency over range 10 mM - 1 μM and 10 mM - 100 nM for [Fe(CN)6]3-/4- and [Fe(CN)6]3-, respectively. Furthermore, the concentration dependency of observed current on two sensor elements shows a relation of monotonic dependency over range 10 mM - 100 nM on two elements.

本誌: 電気学会論文誌E（センサ・マイクロマシン部門誌） Vol.139 No.12 （2019）

本誌掲載ページ: 400-405 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejsmas/139/12/139_400/_article/-char/ja/