電界配向を用いた出芽酵母細胞の生死判別法

カテゴリ: 論文誌(論文単位)

グループ名: 【E】センサ・マイクロマシン部門

発行日: 2020/12/01

タイトル(英語): Electro-orientation-based Viability Assay of Budding Yeast Cells

著者名: 須加　実（富山大学大学院学術研究部工学系），寺西　美里（富山大学大学院学術研究部工学系），篠原　寛明（富山大学大学院学術研究部工学系）

著者名(英語): Minoru Suga (Faculty of Engineering, Academic Assembly, University of Toyama), Misato Teranishi (Faculty of Engineering, Academic Assembly, University of Toyama), Hiroaki Shinohara (Faculty of Engineering, Academic Assembly, University of Toyama)

キーワード: 電界配向，交流電界，酸化インジウムスズ電極，生死判別法，出芽酵母　　electro-orientation，AC electric field，indium-tin-oxide electrode，viability assay，Saccharomyces cerevisiae

要約(英語): A rapid and easy viability assay of budding yeast cells using the electro-orientation (EO) phenomenon in an AC electric field was developed without any reagents. Saccharomyces cerevisiae cell suspension was simply sandwiched between two indium-tin-oxide plate electrodes. The EO directions of each cell indicated whether the cells were alive or dead at a narrow frequency band of 10-20 MHz. By observing through the transparent electrodes, the living cells were appeared as perfect circule shape when oriented parallel to the electric field line, while the dead cells were appeared as snowman and egg shapes when oriented perpendicular to the field.

本誌: 電気学会論文誌E（センサ・マイクロマシン部門誌） Vol.140 No.12 （2020）

本誌掲載ページ: 380-381 p

原稿種別: 研究開発レター／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejsmas/140/12/140_380/_article/-char/ja/