細胞外カルシウムイオンの除去・導入に対する経上皮電気抵抗変化の評価

カテゴリ: 論文誌(論文単位)

グループ名: 【E】センサ・マイクロマシン部門

発行日: 2022/02/01

タイトル(英語): Evaluation of Trans-epithelial Electrical Resistance by Removal and Replenishment of Extracellular Ca2+

著者名: 髙田　裕司（京都大学大学院工学研究科），上野　遼平（京都大学大学院工学研究科），Ramin Banan Sadeghian（京都大学大学院工学研究科），永沼　香織（パナソニック（株） テクノロジー本部），辻　清孝（パナソニック（株） テクノロジー本部），藤本　和也（京都大学大学院工学研究科），横川　隆司（京都大学大学院工学研究科）

著者名(英語): Yuji Takata (Department of Micro Engineering, Kyoto University), Ryohei Ueno (Department of Micro Engineering, Kyoto University), Ramin Banan Sadeghian (Department of Micro Engineering, Kyoto University), Kaori Naganuma (Innovation, Panasonic Corporation), Kiyotaka Tsuji (Innovation, Panasonic Corporation), Kazuya Fujimoto (Department of Micro Engineering, Kyoto University), Ryuji Yokokawa (Department of Micro Engineering, Kyoto University)

キーワード: 経上皮電気抵抗，細胞外カルシウムイオン，細胞極性，細胞間結合，Microphysiological systems (MPS)，腎近位尿細管上皮細胞　　trans-epithelial electrical resistance (TEER)，extracellular Ca2+，cell polarity，cell junction，microphysiological systems (MPS)，renal proximal tubule epithelial cell (RPTEC)

要約(英語): We developed a bi-channel microfluidic device integrated with a four-probe electrode system to measure the trans-epithelial electrical resistance (TEER). Time course of TEER was monitored when the extracellular Ca2+ was removed and replenished both from the upper and lower channels. We showed that tight junctions were disrupted rapidly once Ca2+ was removed from the basal side. However, when Ca2+ was removed from the apical side such disruption progressed slowly. Upon replenishing Ca2+ content at the basal side the TEER recovery pace was also faster than the case of replenishing it at the apical side. We conclude that cell polarity needs to be taken into account during the process of Ca2+ removal/replacement in explaining the dynamics of tight junction disruption/recovery. These results indicate that our approach can be utilized in detecting cell polarity in real time and low invasion.

本誌: 電気学会論文誌E（センサ・マイクロマシン部門誌） Vol.142 No.2 （2022）

本誌掲載ページ: 21-28 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejsmas/142/2/142_21/_article/-char/ja/