iPS細胞胚様体の局所刺激を目指したマイクロデバイスの開発

カテゴリ: 論文誌(論文単位)

グループ名: 【E】センサ・マイクロマシン部門

発行日: 2022/12/01

タイトル(英語): Microfluidic Device for Differentiation Induction of iPS Cells-derived Embryoid Bodies with Local Chemical Stimulation

著者名: 楠　直人（香川大学），小長谷　周平（京都大学　iPS細胞研究所　増殖分化機構研究部門），西田　光徳（タツモ（株）），佐藤　成弘（タツモ（株）），高尾　英邦（香川大学），下川　房男（香川大学），寺尾　京平（香川大学）

著者名(英語): Naoto Kusunoki (Kagawa University), Shuhei Konagaya (Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University), Mitsunori Nishida (TAZMO Co., Ltd.), Shigehiro Sato (TAZMO Co., Ltd.), Hidekuni Takao (Kagawa University), Fusao Shimokawa (Kagawa University), Kyohei Terao (Kagawa University)

キーワード: ヒト人工多能性幹細胞，マイクロウェル，胚様体，細胞分化，局所刺激_x000D_　　human induced pluripotent stem cells，microwell，embryoid body，cell differentiation，local stimulation

要約(英語): This paper reports a microfluidic device that allows us to measure the effect of local differentiation stimulation on embryoid bodies for the purpose of elucidating the role of the cell-cell interaction of iPS cells in differentiation induction. We developed the local stimulation device having two chambers separated by thin polydimethyl siloxane (PDMS) membrane with through-holes of the diameter smaller than single cell. Embryoid bodies of iPS cells are formed on the membrane and seal the holes, separating the aqueous environments of 2 chambers without leakage. This configuration brings localized exposure to chemicals for differentiation induction at the area defined by the holes. In this paper, we demonstrated the function of local stimulation using fluorescent solution.

本誌: 電気学会論文誌E（センサ・マイクロマシン部門誌） Vol.142 No.12 （2022）

本誌掲載ページ: 310-315 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejsmas/142/12/142_310/_article/-char/ja/