味覚センサの辛味測定のためのグルコース酸化酵素標識抗体を用いた前処理法の基礎検討

カテゴリ: 論文誌(論文単位)

グループ名: 【E】センサ・マイクロマシン部門

発行日: 2023/05/01

タイトル(英語): A Basic Study of Pretreatment Method Using Antibody Modified with Glucose Oxidase for Pungent-Taste Measurement of Taste Sensor

著者名: 平山　裕貴（九州大学大学院システム情報科学府），小野寺　武（九州大学大学院システム情報科学府）

著者名(英語): Yuki Hirayama (Graduate of information Science and Electrical Engineering, Kyushu University), Takeshi Onodera (Graduate of information Science and Electrical Engineering, Kyushu University)

キーワード: 辛味，ピペリン，味覚センサ，グルコースオキシダーゼ，ポリクローナル抗体　　pungent taste，piperine，taste sensor，glucose oxidase，polyclonal antibody

要約(英語): A polyclonal antibody against the analog of piperine, which is a typical pungent taste substance, was produced, and its binding ability to piperine was evaluated using a surface plasmon resonance sensor. The antibody was labeled with glucose oxidase (GOx), and the hydrogen ions produced by the enzymatic reaction were measured at a lipid polymer membrane electrode for sourness in a taste sensor. The results showed that piperine could be detected by that polyclonal antibody; the response of the sourness sensor increased in dependence on the GOx-labeled antibody concentration when the GOx of GOx-labeled antibody was reacted with glucose. Thus, it was shown that uncharged pungent compounds can be converted into hydrogen ions. Pungent taste can be measured by the taste sensor using GOx-labeled antibodies, and GOx-labeled antibodies can be used as a pretreatment.

本誌: 電気学会論文誌E（センサ・マイクロマシン部門誌） Vol.143 No.5 （2023） 特集：令和4年度センサ・マイクロマシン部門総合研究会

本誌掲載ページ: 77-81 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejsmas/143/5/143_77/_article/-char/ja/