ヒト臍帯静脈内皮細胞をもちいたオンチップ血管網の長期培養に対する形態と機能の評価

カテゴリ: 論文誌(論文単位)

グループ名: 【E】センサ・マイクロマシン部門

発行日: 2023/05/01

タイトル(英語): Evaluating Morphology and Functionality of On-Chip Vascular Network Derived from Human Umbilical Vein Endothelial Cells (HUVECs) during Long-Term Culture

著者名: 北田　敦也（京都大学大学院工学研究科），Maneesha Shaji（京都大学大学院工学研究科），入佐　大河（京都大学大学院工学研究科），藤本　和也（京都大学大学院工学研究科），Stanislav L. Karsten（京都大学大学院工学研究科），横川　隆司（京都大学大学院工学研究科）

著者名(英語): Atsuya Kitada (Department of Micro Engineering, Kyoto University), Maneesha Shaji (Department of Micro Engineering, Kyoto University), Taiga Irisa (Department of Micro Engineering, Kyoto University), Kazuya Fujimoto (Department of Micro Engineering, Kyoto University), Stanislav L. Karsten (Department of Micro Engineering, Kyoto University), Ryuji Yokokawa (Department of Micro Engineering, Kyoto University)

キーワード: マイクロ流体デバイス，長期培養，三次元培養，血管網，Microphysiological systems (MPS)　　microfluidic devices，long-term culturing，3D tissue culture，vascular network，Microphysiological systems (MPS)

要約(英語): Oxygen and nutrient supply provided via the vascular system is essential for organ development and tissue metabolism. Recent advances in stem cell technologies have led to the development of complex 3D tissue culture systems. However, direct supply of nutrients and oxygen by simple diffusion fails to reach inner areas resulting in impaired maturity of these complex tissues over time. In an attempt to alleviate this problem, 3D tissues are integrated with vascular networks (VNs) on a chip to ensure appropriate oxygen and nutrient supply. Such an in vitro vascular system must maintain a stable, robust, and perfusable network without degradation during entire culture period that may easily exceed several weeks. Current protocols for generation of VNs provide no information on the changes in these parameters in the long term. Here, using human umbilical vein endothelial cells, we generated a vascular network on a microfluidic chip and evaluated its morphology and function for 35 days. We demonstrate network stability, preservation of lumen structure and robust perfusability with negligible increase in permeability over time.

本誌: 電気学会論文誌E（センサ・マイクロマシン部門誌） Vol.143 No.5 （2023） 特集：令和4年度センサ・マイクロマシン部門総合研究会

本誌掲載ページ: 82-88 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejsmas/143/5/143_82/_article/-char/ja/