交流ナノポア法による単一ナノ粒子のインピーダンス計測とウイルスセンシングへの応用

カテゴリ: 論文誌(論文単位)

グループ名: 【E】センサ・マイクロマシン部門

発行日: 2023/07/01

タイトル(英語): Impedance Measurement of Single Nanoparticles by AC Nanopore Method and Application to Virus Sensing

著者名: 山本　貴富喜（東京工業大学 工学院 機械系），橘田　和樹（東京工業大学 工学院 機械系），坂本　まあみ（東京工業大学 工学院 機械系），早川　渓（（株）デンソー マテリアル研究部），糠塚　明（（株）デンソー マテリアル研究部），加納　一彦（（株）デンソー マテリアル研究部）

著者名(英語): Takatoki Yamamoto (Department of Mechanical Engineering, Tokyo Institute of Technology), Kazuki Kitta (Department of Mechanical Engineering, Tokyo Institute of Technology), Maami Sakamoto (Department of Mechanical Engineering, Tokyo Institute of Technology), Kei Hayakawa (Material Research and Innovation Division, DENSO CORPORATION), Akira Nukazuka (Material Research and Innovation Division, DENSO CORPORATION), Kazuhiko Kano (Material Research and Innovation Division, DENSO CORPORATION)

キーワード: ナノポア，インピーダンス，交流，ロックインアンプ，ウイルス，ナノ粒子　　nanopore，impedance，alternative current，lock-in amp，virus，nanoparticle

要約(英語): Nanopore-based sensing methods are valuable tools for solution-based single-particle analysis of many biomolecules and particles that play important roles in life and medicine, such as nucleic acids, proteins, viruses, and exosomes. The conventional nanopore method is based on direct current measurement, and the available information on particle size and zeta potential is limited. Here, we developed an alternating current-based nanopore method using lock-in measurement to overcome the longstanding and conflicting limitations of both low noise and wide bandwidth, thereby demonstrating impedance measurement of a single nanoparticle. We determined the particle size, zeta potential, and electric capacitance from the frequency responses of various particles ranging in diameter from 100 nm to several micrometers. Our nanopore method was highly accurate, identifying the type of virus with over 85% sensitivity using single-particle measurement and machine learning. This method is expected to have a variety of applications such as sensing of various nanoparticles and characterization of single nanoparticles.

本誌: 電気学会論文誌E（センサ・マイクロマシン部門誌） Vol.143 No.7 （2023） 特集：第39回「センサ・マイクロマシンと応用システム」シンポジウム受賞論文

本誌掲載ページ: 176-184 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejsmas/143/7/143_176/_article/-char/ja/