二次元電気化学センサLAASを用いたアセチルコリンエステラーゼ測定の研究

カテゴリ: 論文誌(論文単位)

グループ名: 【E】センサ・マイクロマシン部門

発行日: 2023/08/01

タイトル(英語): Measurement of Acetylcholinesterase Using a Two-dimensional Electrochemical Sensor; LAAS

著者名: 宮入　彬承（埼玉大学 大学院理工学研究科），長谷川　有貴（埼玉大学 大学院理工学研究科），内田　秀和（埼玉大学 大学院理工学研究科）

著者名(英語): Akitsugu Miyairi (Graduate School of Science and Engineering, Saitama University), Yuki Hasegawa (Graduate School of Science and Engineering, Saitama University), Hidekazu Uchida (Graduate School of Science and Engineering, Saitama University)

キーワード: ショットキー接合，電気化学イメージングセンサ，マイクロアレイ，LAAS，創薬スクリーニング，アセチルコリンエステラーゼ　　Schottky junction，electrochemical imaging sensor，micro array，LAAS，drug screening，acetylcholinesterase

要約(英語): In this study, we measured the enzymatic activity of acetylcholinesterase (AChE) using the Light Addressable Amperometric Sensor (LAAS), an electrochemical sensor that can easily measure redox current values of multiple samples.Acetylthiocholine (ATCh) was used as a substrate. ATCh is hydrolyzed by AChE and releases electrons, making it possible to measure current values in accordance with the amount of substrate using LAAS. When KCl solution was used as the supporting electrolyte and an Ag/AgCl reference electrode as the counter electrode, a correlation between substrate concentration and reaction was confirmed in the range of 1μM to 10mM. Malathion was then used as an inhibitor of AChE. Malathion phosphorylates and inactivates AChE, which prevents substrate hydrolysis and is expected to decrease the current value. Experimental results showed a decrease in sensor response correlated with inhibitor concentration.

本誌: 電気学会論文誌E（センサ・マイクロマシン部門誌） Vol.143 No.8 （2023）

本誌掲載ページ: 219-224 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejsmas/143/8/143_219/_article/-char/ja/