微小管を用いた高感度タウタンパク質検出法

カテゴリ: 全国大会

論文No: 3-108

グループ名: 【全国大会】平成25年電気学会全国大会論文集

発行日: 2013/03/05

タイトル(英語): Development of a highly sensitive microtubule based tau detection method

著者名: オラゾフイスラム (東京大学),タルハンチャータイ (東京大学),横川 隆司(京都大学),カースタンスタニスラフ (NeuroInDx,Inc.),藤田 博之(東京大学)

著者名(英語): Yslam Orazov(The University of Tokyo),Cagatay Tarhan(The University of Tokyo),Ryuji Yokokawa(Kyoto university),Stanislav Karsten(NeuroInDx,Inc.),Hiroyuki Fujita(The University of Tokyo)

キーワード: 微小管|キネシン|タウタンパク質|神経伝達

要約(日本語): Microtubule (MT)-based intraneuronal transport is often altered in several neurological diseases. To understand complex molecular processes underlying MT-based neuronal transport, e.g. stabilization of MT by its associated proteins, a reliable and sensitive in vitro assay is required. Such assay would permit analysis of various conditions and components affecting MT-based transport, facilitating identification of drugs modulating disease course, and ultimately resulting in the development of new theraupetic approaches. This paper focuses on improving the protein-MT interaction assay based on reconstructed kinesin/microtubule system. Different MT immobilization processes were compared on tau protein detection. The system using MTs suspended between microstructures provided higher statistical significance.

原稿種別: 日本語

PDFファイルサイズ: 520 Kバイト