酵素光測定法による生理活性物質の可視化

カテゴリ: 全国大会

論文No: 1-120

グループ名: 【全国大会】平成26年電気学会全国大会論文集

発行日: 2014/03/05

タイトル(英語): Visualization of Spatially Distributed Bioactive Molecules using Enzyme-Linked Photo Assay

著者名: 恩 本八百(豊橋技術科学大学)

著者名(英語): Naohiro Hozumi()

キーワード: 神経伝達物質|酵素光測定技術|画像処理|生理活性物質

要約(日本語): In this paper, we propose a new simple device for this purpose by using a membrane in which a specific enzyme is immobilized. The membrane produces fluorescence after combining with a specific metabolite. The membrane, on which the tissue is mounted, is deposited on a quartz substrate that is used as a light guide for the excitation UV light. Metabolite release is observed by a CCD camera from the opposite side of the substrate. The paper also describes the reduction of background fluorescence by means of image processing technique. We increased the number of UV light to two so that the background noise can be eliminated. Finally the release of ATP from specific layers in rat cerebellum was successfully observed. In the future, it is to be believed that instead of manual tuning, we might produce a program which using auto tuning for a more precise image processing.

原稿種別: 日本語

PDFファイルサイズ: 294 Kバイト