Electrochemical Microdevice for Highly Sensitive Coulometric Detection of Proteins

カテゴリ: 全国大会

論文No: 3-120

グループ名: 【全国大会】平成29年電気学会全国大会論文集

発行日: 2017/03/05

タイトル(英語): Electrochemical Microdevice for Highly Sensitive Coulometric Detection of Proteins

著者名: Isa Anshori(筑波大学),Hiroaki Suzuki(筑波大学)

著者名(英語): Isa Anshori(University of Tsukuba),Hiroaki Suzuki(University of Tsukuba)

キーワード: クーロメトリー|メタライゼーション|マイクロフルイディクス|プラグ|タンパク質検出

要約(日本語): Highly sensitive detection of proteins was realized using coulometric detection coupled with metallization. Two microfluidic channels were used to accommodate electrodes for the oxidation of an enzymatic reaction product and deposition of silver that occur simultaneously at the mixed potential. Enzyme-linked immunosorbent assay (ELISA) was conducted and hydrogen peroxide (H2O2) was oxidized on the electrode. The amount of deposited silver was measured by coulometry. By replacing the glucose plug with fresh ones to produce H2O2 additionally, the signal could be increased. Estimated detection limit of 0.4 ng/ml was achieved using the device.

原稿種別: 英語

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