Time Dependence of Nonlinear Dielectric Responses in Yeast Cells at Various Concentrations

カテゴリ: 論文誌(論文単位)

グループ名: 【A】基礎・材料・共通部門

発行日: 2012/11/01

タイトル(英語): Time Dependence of Nonlinear Dielectric Responses in Yeast Cells at Various Concentrations

著者名: Kazuaki Mizuyama (Graduate School of Engineering, Osaka City University), Masafumi Muraji (Graduate School of Engineering, Osaka City University)

著者名(英語): Kazuaki Mizuyama (Graduate School of Engineering, Osaka City University), Masafumi Muraji (Graduate School of Engineering, Osaka City University)

キーワード: concentration，nonlinear dielectric response，Saccharomyces cerevisiae，time dependence

要約(英語): We report the time course of nonlinear dielectric responses in living and dead cells of the budding yeast Saccharomyces cerevisiae with changes in their concentrations in suspension. To measure nonlinear dielectric responses, a sinusoidal electric field (1.2V/cm, 14Hz) was applied to the outer electrodes of a 4-terminal system and the response waveform was captured across the inner electrodes. Fourier transform permits determination of the harmonics or other frequencies not present in the exciting waveform. We measured the response waveform for 3 min and showed a relationship between the time course and relative harmonic contents. The results indicated that in living cells, the third harmonic content increased and the fifth harmonic content decreased with increasing concentration. The relative harmonic content of dead cells, however, remained similar regardless of the yeast cell concentration. The differences between living and dead cells became more conspicuous as the concentration increased. Observation of the responses permitted us to distinguish between living and dead cells.

本誌: 電気学会論文誌A（基礎・材料・共通部門誌） Vol.132 No.11 （2012） 特集：放電現象・新素材とその応用技術

本誌掲載ページ: 993-996 p

原稿種別: 論文／英語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejfms/132/11/132_993/_article/-char/ja/