ナノ秒パルス強電界による細胞死誘導とパルス幅依存性

カテゴリ: 論文誌(論文単位)

グループ名: 【A】基礎・材料・共通部門

発行日: 2017/06/01

タイトル(英語): Cell Death induced by Nanosecond Pulsed Electric Fields and its Dependence on Pulse Duration

著者名: 安達　隆太（熊本大学大学院自然科学研究科），畑山　翔太（熊本大学大学院自然科学研究科），大西　伸明（熊本大学大学院自然科学研究科），勝木　淳（熊本大学パルスパワー科学研究所），和田　敏明（熊本大学大学院自然科学研究科），安部　恵祐（大分大学全学研究推進機構）

著者名(英語): Ryuta Andachi (Graduate School of Science and Technology, Kumamoto University), Shota Hatayama (Graduate School of Science and Technology, Kumamoto University), Nobuaki Ohnishi (Graduate School of Science and Technology, Kumamoto University), Sunao Katsuki (Institute of Pulsed Power Science, Kumamoto University), Toshiaki Wada (Graduate School of Science and Technology, Kumamoto University), Keisuke Abe (Research Promotion Institute, Oita University)

キーワード: ナノ秒強電界パルス，パルス幅，プログラム細胞死，カルシウムイオン，細胞膜障害，タンパク質発現　　nanosecond pulsed electric field，pulse duration，programed cell death，Ca ion，membrane damage，protein expression

要約(英語): We discuss the death process of human cervical cancer cells HeLa S3 induced by two types of non-thermal intense electrical pulses of different durations and amplitudes, 120 ns-long, 12.5 kV/cm and 5 ns-long, 250 kV/cm. WST-8 assay was used to evaluate the cell viability. Expression of three proteins associated with the stress response, Caspase 3, ASK1 and BAX, were analyzed by using the antibody assay and flowcytometry. Apoptosis activity was evaluated by means of the TUNEL assay and flowcytometry. Also, Ca2+ dependences of the death process and the protein expression were explored by using the metal ion chelating agent, EDTA. Our experiments demonstrated that 5 ns and 120 ns pulses give quite different biological stresses leading to the different death process and the protein expressions. The cell death induced by 120 ns pulses are dependent on the presence of Ca2+ and accompanied by expression of proteins associated with apoptosis and stress response. Conversely, the protein expression induced by 5 ns pulses was independent of the presence of Ca2+, whereas the death was Ca2+ dependent.

本誌: 電気学会論文誌A（基礎・材料・共通部門誌） Vol.137 No.6 （2017） 特集：パルスパワーの産業応用

本誌掲載ページ: 320-327 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejfms/137/6/137_320/_article/-char/ja/