微小電極アレイを用いた上丘スライス自発活動の培養計測

カテゴリ: 論文誌(論文単位)

グループ名: 【C】電子・情報・システム部門

発行日: 2011/07/01

タイトル(英語): Recording of Spontaneous Activity in Cultured Superior Colliculus Slices Using Microelectrode Array

著者名: 廣田　晋也（東京大学大学院　新領域創成科学研究科），井上　康輔（東京大学大学院　新領域創成科学研究科），森口　裕之（東京大学大学院　新領域創成科学研究科），高山　祐三（東京大学大学院　情報理工学系研究科），神保　泰彦（東京大学大学院　新領域創成科学研究科）

著者名(英語): Shinya Hirota (Graduate School of Frontier Sciences, University of Tokyo), Kousuke Inoue (Graduate School of Frontier Sciences, University of Tokyo), Hiroyuki Moriguchi (Graduate School of Frontier Sciences, University of Tokyo), Yuzo Takayama (Graduate School of Information Science and Technology, University of Tokyo), Yasuhiko Jimbo (Graduate School of Frontier Sciences, University of Tokyo)

キーワード: 微小電極アレイ，上丘，スライス培養，自発活動　　Microelectrode Array (MEA), Superior Colliculus, Slice Culture, Spontaneous Activity, spike, Local Field Potential (LFP)

要約(英語): Retino-tectal projection is one of the pathways in visual information processing. During development, spontaneous electrical activity of superior colliculus (SC) probably plays an important role for proper network formation. In this study, SC slices were taken from new-born rats and cultured on microelectrode-array (MEA) substrates. Spontaneous activities of the cultured SC were recorded for four weeks. During the first week, most of the cultured SC slices generated sporadic spikes. These sporadic spikes gradually changed into periodic synchronized bursts. Spatially organized synchronized activity produced low frequency signals (local field potential: LFP). All of the test slices showed clear LFPs within three weeks. Large negative peaks were observed in the middle layer of the slices, while positive signals were obtained around the deep layers. Current source density (CSD) analysis revealed that middle layers acted as a current source and surface and deep layers were the sinks. These results suggested that layered structures of SC were well-maintained under the culture conditions and the cells in the middle layers played dominant roles in generating these periodic synchronized spontaneous activities.

本誌: 電気学会論文誌C（電子・情報・システム部門誌） Vol.131 No.7 （2011） 特集：平成22年電気学会電子・情報・システム部門大会

本誌掲載ページ: 1355-1360 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejeiss/131/7/131_7_1355/_article/-char/ja/