集積化マイクロ培養チャンバを用いた神経細胞移植モデルの構築

カテゴリ: 論文誌(論文単位)

グループ名: 【C】電子・情報・システム部門

発行日: 2012/07/01

タイトル(英語): Neural Transplantation Model Using Integration Co-culture Chamber

著者名: 榛葉　健太（東京大学大学院　新領域創成科学研究科），斎藤　淳史（東京大学大学院　新領域創成科学研究科），武内　彬正（東京大学大学院　新領域創成科学研究科），高山　祐三（東京大学大学院　情報理工学系研究科），小谷　潔（東京大学大学院　新領域創成科学研究科），神保　泰彦（東京大学大学院　新領域創成科学研究科）

著者名(英語): Kenta Shimba (Graduate School of Frontier Sciences, The University of Tokyo), Atsushi Saito (Graduate School of Frontier Sciences, The University of Tokyo), Akimasa Takeuchi (Graduate School of Frontier Sciences, The University of Tokyo), Yuzo Takayama (Graduate School of Information Science and Technology, The University of Tokyo), Kiyoshi Kotani (Graduate School of Frontier Sciences, The University of Tokyo), Yasuhiko Jimbo (Graduate School of Frontier Sciences, The University of Tokyo)

キーワード: 微小電極アレイ基板，神経回路，幹細胞，共培養，再生医療，微細加工技術　　microelectrode array，neuronal network，stem cell，co-culture，tissue engineering，microfabrication technique

要約(英語): Regenerative medicine is a promising therapy for injuries and diseases of the central nervous system (CNS). Implantation of stem cell-derived neurons into the recipient tissue is one of the key processes of the therapy. How the implanted cells establish functional connections with the intact neurons, and whether the established connections are maintained stably for a long time, remain unknown. Here, we report a novel co-culture device for visualizing interconnections between primary and differentiated neuronal cultures, and long-term monitoring of neuronal activity. A circular micro-chamber surrounded by another chamber is aligned on a microelectrode array (MEA). These chambers are interconnected through 36 micro-tunnels. Stem cell-derived neurons were cultured in the inner circular chamber, and primary neurons taken from mouse cortices were cultured in the surrounding chamber. Neurites outgrew into the micro-tunnels from both primary and differentiated neurons. The immunofluorescence images indicate that synaptic connections are formed between them. Propagation of electrical activity was observed 6 days after starting co-culture. More than half of the spontaneous activity was initiated from primary neurons, and probability of activity propagation to the stem cell-derived neurons gradually increased with culture days. These results suggest that our device is feasible for long-term monitoring of interaction between stem cell-derived cells and the recipient tissue.

本誌: 電気学会論文誌C（電子・情報・システム部門誌） Vol.132 No.7 （2012） 特集：平成23年電気学会電子・情報・システム部門大会

本誌掲載ページ: 1072-1078 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejeiss/132/7/132_1072/_article/-char/ja/