微小流体デバイスを用いた可搬型高速遺伝子検査システム

カテゴリ: 論文誌(論文単位)

グループ名: 【C】電子・情報・システム部門

発行日: 2014/04/01

タイトル(英語): Portable Microfluidic System for Rapid Genetic Testing

著者名: 永井　秀典（産業技術総合研究所），渕脇　雄介（産業技術総合研究所）

著者名(英語): Hidenori Nagai (National Institute of Advanced Industrial Science and Technology), Yusuke Fuchiwaki (National Institute of Advanced Industrial Science and Technology)

キーワード: ポリメラーゼ連鎖反応（PCR），遺伝子検査，ポイント・オブ・ケア・テスト（POCT），微小流体デバイス　　polymerase chain reaction (PCR)，genetic testing，point of care testing (POCT)，microfluidics

要約(英語): Polymerase chain reaction (PCR) is a key technology of genetic testing, but the long thermal cycling time has been the problem to apply the rapid diagnosis of pathogenic organisms and cancer. We describe a simple, portable, and rapid genetic testing system based on ultrafast segment-flow and fluorescent detection. Especially, we improved the flow-through PCR to accelerate the thermal cycling time and simplify the operation for the practical use. The PCR solution was injected with a small volume about several microliter, and flowed as segment-flow by an air syringe. The segment of PCR solution was moved in a serpentine microchannel on a microfluidic device over two temperature zones for denaturation and annealing/extension reactions. The base plate formed upon the serpentine microchannel pattern was made of cyclo-olefin polymer (COP) and covered with a thin transparent film. Furthermore, the specialized portable system was developed for the microfluidic device of segment-flow PCR. The heater units, a syringe pump, fluorescence detector, PC were integrated in a suitcase for portable use. Using this portable system, a model microorganism of anthrax and a point mutation of FGFR3 gene were successfully detected within 6.5 min.

本誌: 電気学会論文誌C（電子・情報・システム部門誌） Vol.134 No.4 （2014） 特集：量子ビームによるナノバイオ物理応用技術

本誌掲載ページ: 510-514 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejeiss/134/4/134_510/_article/-char/ja/