神経細胞－オリゴデンドロサイト共培養環境下における活動伝導評価系の構築

カテゴリ: 論文誌(論文単位)

グループ名: 【C】電子・情報・システム部門

発行日: 2014/08/01

タイトル(英語): Neuron-Oligodendrocyte Co-culture Device for Measuring Action Potential Conduction Velocity

著者名: 酒井　洸児（東京大学大学院 新領域創成科学研究科），榛葉　健太（東京大学大学院 新領域創成科学研究科），有松　和之（東京大学大学院 新領域創成科学研究科），小谷　潔（東京大学大学院 新領域創成科学研究科），神保　泰彦（東京大学大学院 新領域創成科学研究科）

著者名(英語): Koji Sakai (Graduate School of Frontier Sciences, The University of Tokyo), Kenta Shimba (Graduate School of Frontier Sciences, The University of Tokyo), Kazuyuki Arimatsu (Graduate School of Frontier Sciences, The University of Tokyo), Kiyoshi Kotani (Graduate School of Frontier Sciences, The University of Tokyo), Yasuhiko Jimbo (Graduate School of Frontier Sciences, The University of Tokyo)

キーワード: 跳躍伝導，オリゴデンドロサイト，共培養，培養神経回路網，培養デバイス，微小電極アレイ　　saltatory conduction，oligodendorocyte，co-culture，neuronal networks，culture device，microelectrode array

要約(英語): Myelination is essential for rapid conduction of action potentials in central nervous system. The loss of myelin leads to impairment of motor and cognitive functions. Remyelination by transplantation of oligodendrocytes (OL) is a potent therapeutic strategy for the demyelinating diseases. However, little is known about how the conduction velocity increases after remyelination. Here, the aim of this study is to develop a culture device for evaluating the change in action potential conduction velocity along axon in co-culture of neurons and OL. We developed the culture device which composed of a culture chamber and a microelectrode array (MEA). The chamber has two neuron culture compartments and four OL culture compartments which are interconnected by four microchannels. Electrodes of the MEA were in alignment with the microchannels. Cortical neurons and OL were cultured separately with a co-culture chamber. OL maturation and axon outgrowth through the microchannels were confirmed by immunofluorescence imaging. After neural activities were recorded, spike sorting was performed to separate spikes into clusters of individual neurons. The propagations of action potentials along axon were detected from spike trains. Then, the conduction velocity increased with culture days. These results suggest that this device can be utilized for evaluating the change in action potential conduction velocity in myelination process.

本誌: 電気学会論文誌C（電子・情報・システム部門誌） Vol.134 No.8 （2014） 特集：産業界における新たな通信・ネットワーク技術の活用

本誌掲載ページ: 1056-1063 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejeiss/134/8/134_1056/_article/-char/ja/