共鳴光ピンセットによる神経細胞接着分子の捕捉

カテゴリ: 論文誌(論文単位)

グループ名: 【C】電子・情報・システム部門

発行日: 2014/08/01

タイトル(英語): Trapping of Neural Cell Adhesion Molecules in Neurons with Resonant Optical Tweezers

著者名: 武田　尚子（産業技術総合研究所 健康工学研究部門／関西学院大学 理工学部 人間システム工学科），工藤　卓（関西学院大学 理工学部 人間システム工学科），田口　隆久（情報通信研究機構 脳情報通信融合研究センター），細川　千絵（産業技術総合研究所 健康工学研究部門）

著者名(英語): Naoko Takeda (Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)/Department of Human System Interaction, School of Science and Technology, Kwansei Gakuin University), Suguru N. Kudoh (Department of Human System Interaction, School of Science and Technology, Kwansei Gakuin University), Takahisa Taguchi (Center for Information and Neural Networks, National Institute of Information and Communications Technology (NICT)), Chie Hosokawa (Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST))

キーワード: 光捕捉，神経細胞接着分子，量子ドット，分散培養，共鳴輻射力　　Optical Trapping，Neural Cell Adhesion Molecule，Quantum dot，Dissociated Culture，Resonant Radiation Force

要約(英語): Living neuronal network in neural systems communicate through synaptic connections. For aiming artificial control of the synaptic transmission in neuronal network, we have applied conventional optical tweezers using a focused near-infrared (NIR) laser. The optical trapping of neural cell adhesion molecules (NCAMs) labeled with quantum-dots (Q-dots) at the plasma membrane of living neuronal cells was achieved at high laser power (>300 mW) in our previous study; therefore, the further reduction of laser power was required. Here, we propose and demonstrate resonant optical tweezers of Q-dots attached to NCAM in a neuron by simultaneous irradiation with non-resonance and resonance laser beams. The optical trapping dynamics of them was revealed by fluorescence correlation spectroscopy (FCS). The transit times of Q-dots attached to NCAM at the focal spot increased with the simultaneous irradiation of NIR laser and resonance laser. These results suggest that the particle motion of Q-dots attached to NCAM was constrained at the focus spot due to optical trapping force enhanced with resonance laser irradiation. Our method is applicable to selective manipulation of signaling molecules in living neurons.

本誌: 電気学会論文誌C（電子・情報・システム部門誌） Vol.134 No.8 （2014） 特集：産業界における新たな通信・ネットワーク技術の活用

本誌掲載ページ: 1071-1077 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejeiss/134/8/134_1071/_article/-char/ja/