ポリピロールを用いた電気化学バイオセンサの機能デザイン

カテゴリ: 論文誌(論文単位)

グループ名: 【C】電子・情報・システム部門

発行日: 2015/02/01

タイトル(英語): Functional Design of Electrochemical Biosensors Using Polypyrrole

著者名: Daluwathu Mulla Gamage Preethichandra（Central Queensland University），小野田　光宣（兵庫県立大学 姫路工学キャンパス 大学院工学研究科）

著者名(英語): Daluwathu Mulla Gamage Preethichandra (Central Queensland University), Mitsuyoshi Onoda (University of Hyogo, Himeji Engineering Campus)

キーワード: ポリピロール，電気化学的重合，アルミナナノ細管，ブドウ糖酸化酵素，酵素吸着，酵素包括　　polypyrrole，electrolysis polymerization，alumina nanotube，glucose oxidase，enzyme adsorption，enzyme entrapment

要約(英語): In order to improve biosensor performance namely, the sensitivity, response time, and linear range values, novel amerometric polypyrrole (PPy) glucose biosensors, achieved by three different immobilizing techniques namely, physical adsorption, coentrapment and a two steps method of coentrapment superimposed with physical adsorption had been studied. Different enzyme loading and material properties resulted in varied immobilizing techniques lead to dissimilarities in characteristics. In this study, Pt coated AnodiscTMs with 0.2 μm maximum pore size were used as electrodes to polymerize PPy at 0.3 mAcm-2 using a solution containing 0.05 mol/l pyrrole and 0.1 M NaPF6. The polymerization time was optimized to 90 s. In the coentrapment method, glucose oxidase (GOx) (1 mg/ml) was added to the monomer solution while an aliquot of GOx (5 μl) was placed on the pre-polymerized electrode for physical adsorption. A combined procedure of these two techniques was used as the last immobilization technique. Physical adsorption method gave a sensitivity of 3 mAcm-2 (mol/l)-1 and a linear range of 0.5-13×10-3 mol/l with a response time of a 3 s. Sensitivity in the case of coentrapment was 3.75 mAcm-2 (mol/l)-1 while it showed a value of 4.45 mAcm-2 (mol/l)-1 in two step immobilization thus giving 25 and 48 % increases respectively. Response times of 9 and 8 s in latter cases reveal the possible repercussion taking place at enzyme immobilization in three dimensional (3-D) PPy matrix and the delay occur in glucose to reach the enzyme. Linear range of the two step method was extended up to 16×10-3 mol/l due to the reinforced enzyme loading.

本誌: 電気学会論文誌C（電子・情報・システム部門誌） Vol.135 No.2 （2015） 特集：フレキシブルフィルムデバイスのための材料・プロセスと評価技術

本誌掲載ページ: 174-180 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejeiss/135/2/135_174/_article/-char/ja/