培養心室筋モデルにおけるリエントリー様旋回波の再現と伝播パターン評価

カテゴリ: 論文誌(論文単位)

グループ名: 【C】電子・情報・システム部門

発行日: 2020/07/01

タイトル(英語): Evaluating Propagation Pattern During Reentry-like Activity of Cultured Cardiomyocytes

著者名: 岡本　美優（東京大学大学院 工学系研究科），榛葉　健太（東京大学大学院 工学系研究科），加茂野　照大（東京大学大学院 工学系研究科），小谷　潔（東京大学 先端科学技術研究センター），神保　泰彦（東京大学大学院 工学系研究科）

著者名(英語): Miyu Okamoto (School of Engineering, The University of Tokyo), Kenta Shimba (School of Engineering, The University of Tokyo), Akihiro Kamono (School of Engineering, The University of Tokyo), Kiyoshi Kotani (Research Center for Advanced Science and Technology, The University of Tokyo), Yasuhiko Jimbo (School of Engineering, The University of Tokyo)

キーワード: リエントリー，不整脈，心筋細胞，微小電極アレイ　　Reentry，arrhythmia，cardiomyocytes，microelectrode array

要約(英語): Heart failure is one of frequent diseases for the elderly, and it is required to unveil the pathogenic mechanism of reentry, a type of lethal arrhythmia. Although fibrosis can cause arrythmia, little is known about how fibrosis disrupts normal cardiac function, and then causes arrythmia. In this study, we aimed to model reentry-like activity in vitro for elucidating the mechanism of reentry. First, rat cardiomyocytes were purified with a glucose-free medium, and cultured on microelectrode arrays. Extracellular potential was measured with or without lidocaine, a proarrhythmic reagent, on day 6-7. Reentry-like activities were observed from three out of five samples in presence and absence of lidocaine. We evaluated transitions between reentry and normal propagation. Activity pattern gradually changed from normal propagation to reentry, but that did rapidly from reentry to normal propagation, suggesting different mechanisms between start and end of reentry. Furthermore, changes in propagation pattern during reentry suggested that delay prolongation in partial area can terminate reentry-like activity in vitro. Overall, our system is suitable for elucidating pathogenic mechanisms of reentry.

本誌: 電気学会論文誌C（電子・情報・システム部門誌） Vol.140 No.7 （2020） 特集：2019年電子・情報・システム部門大会

本誌掲載ページ: 730-736 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejeiss/140/7/140_730/_article/-char/ja/