表面プラズモン共鳴免疫センサを用いたトウガラシ果実中のカプサイシノイド検出

カテゴリ: 論文誌(論文単位)

グループ名: 【E】センサ・マイクロマシン部門

発行日: 2016/06/01

タイトル(英語): Detection of Capsaicinoids in Capsicum Fruit Using Surface Plasmon Resonance Immunosensor

著者名: 小野寺　武（九州大学 味覚・嗅覚センサ研究開発センター），田　勝煕（九州大学大学院システム情報科学研究院），矢田部　塁（九州大学 味覚・嗅覚センサ研究開発センター），都甲　潔（九州大学 味覚・嗅覚センサ研究開発センター／九州大学大学院システム情報科学研究院）

著者名(英語): Takeshi Onodera (Research and Development Center for Taste and Odor Sensing, Kyushu University), Shengxi Tian (Graduate School and Faculty of information Science and Electrical Engineering, Kyushu University), Rui Yatabe (Research and Development Center for Taste and Odor Sensing, Kyushu University), Kiyoshi Toko (Research and Development Center for Taste and Odor Sensing, Kyushu University/Graduate School and Faculty of information Science and Electrical Engineering, Kyushu University)

キーワード: カプサイシン，カプサイシノイド，表面プラズモン共鳴，辛味，抗原抗体反応，免疫センサ　　capsaicin，capsaicinoid，surface plasmon resonance，pungency，antigen-antibody interaction，immunosensor

要約(英語): Detection method of capsaicinoids such as capsaicin, dihydrocapsaicin and nordihydrocapsaicin was developed using anti-homovalic acid-Concholepas concholepas hemocyanin (CCH) polyclonal antibodies and surface plasmon resonance (SPR) sensor with indirect competitive assay method. Extraction procedure of capsaicinoids from Capsicum fruit was also developed. As a result, limits of detection for capsaicin, dihidrocapsaicin and nordihydrocapsaicin were 27 ppb, 35 ppb and 50 ppb, respectively. The reduction of responses in SPR measurements with indirect competitive method for recovered samples of sweet peppers such as Bell pepper and Shishitougarashi were not observed. Nine kinds of hot peppers (eg. Habanero, Trinidad scorpion), which have different Scovile heat unit (SHU), were also tested. The reductions of sensor responses for the hot peppers were observed. It is likely that samples extracted from Capsicum fruit of the hot peppers contained capsaicinoids. It was found that the responses of the SPR immunosensor depended on SHU of each hot pepper.

本誌: 電気学会論文誌E（センサ・マイクロマシン部門誌） Vol.136 No.6 （2016） 特集：第32回「センサ・マイクロマシンと応用システム」シンポジウム受賞論文特集

本誌掲載ページ: 218-223 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejsmas/136/6/136_218/_article/-char/ja/