誘電泳動速度計測によるがん細胞の誘電特性の基礎検討

カテゴリ: 論文誌(論文単位)

グループ名: 【E】センサ・マイクロマシン部門

発行日: 2021/04/01

タイトル(英語): Basic Study of Dielectric Properties of Cancer Cells by Dielectrophoretic Velocimetry

著者名: 郭　宇桐（東京都立大学），八木　一平（東京都立大学），内田　諭（東京都立大学），杤久保　文嘉（東京都立大学），高野　雅代（（株）AFIテクノロジー），脇坂　嘉一（（株）AFIテクノロジー），円城寺　隆治（（株）AFIテクノロジー）

著者名(英語): Yutong Guo (Tokyo Metropolitan University), Ippei Yagi (Tokyo Metropolitan University), Satoshi Uchida (Tokyo Metropolitan University), Fumiyoshi Tochikubo (Tokyo Metropolitan University), Masayo Takano (AFI Corporation), Yoshikazu Wakizaka (AFI Corporati

キーワード: 誘電泳動，速度計測，Jurkat細胞，誘電特性，Clausius-Mossotti関数　　dielectrophoresis，velocimetry，Jurkat cells，dielectric property，Clausius-Mossotti function

要約(英語): Cell separation technique is essential for the detection of circulating tumor cells in blood and for enrichment of stem cell in regenerative medicine. However, conventional methods require cell labeling such as antibodies, which may damage the cells during the operation. Therefore, dielectrophoresis (DEP), a "label-free" separation technique that eliminates the effects on cells, has been attracting attention. In this paper, we investigated the dielectric properties of Jurkat cells derived from human T-cell leukemia cells by dielectrophoretic velocimetry (DEPVM). An equivalent circuit model of the cell was constructed and the frequency characteristics of the voltage of each layer of the cell were calculated. As a result, it was shown that the live and dead cells showed opposite dielectrophoretic forces in the region below 10 kHz, indicating the possibility of separating them. The agreement between the theoretical calculations and the experimental results shows the validity of the equivalent circuit model.

本誌: 電気学会論文誌E（センサ・マイクロマシン部門誌） Vol.141 No.4 （2021）

本誌掲載ページ: 108-112 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejsmas/141/4/141_108/_article/-char/ja/