ニューロン細胞外電位の安定的計測を目的とするSiN多孔膜を介した階層型共培養

カテゴリ: 論文誌(論文単位)

グループ名: 【E】センサ・マイクロマシン部門

発行日: 2021/07/01

タイトル(英語): Back-to-back Layered Co-culture on a SiN Porous Membrane for Stable Measurement of Neuronal Extracellular Potentials

著者名: 吉田　悟志（九州工業大学 大学院生命体工学研究科），安田　隆（九州工業大学 大学院生命体工学研究科）

著者名(英語): Satoshi Yoshida (Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology), Takashi Yasuda (Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology)

キーワード: 微小電極アレイ，細胞外電位計測，共培養，ニューロン，アストロサイト　　microelectrode array，extracellular potential measurement，co-culture，neuron，astrocyte

要約(英語): We developed a novel technique for measuring neuronal extracellular potentials in a neuron-astrocyte co-culture system using a SiN (silicon nitride) membrane having microelectrodes and microholes. An 8×8 microelectrode array was formed on one side of a 1 μm-thick SiN membrane, and multiple microholes of 5 μm in diameter were opened through the membrane. Then, neurons were cultured on the microelectrodes after astrocytes were cultured on the back side of the membrane. The neuronal spikes measured in this novel back-to-back co-culture method were compared with those in the conventional method in which both of neurons and astrocytes were co-cultured on the microelectrodes. In the conventional method, formation and enlargement of cellular aggregates made neurons out of touch with microelectrodes, and posed measurement of a small number of spikes. On the other hand, our novel method permitted the firm contact between neurons and microelectrodes and the cell-to-cell interaction through microholes, and therefore neuronal spikes increased drastically with culture time, which led to stable measurement of neuronal extracellular potentials.

本誌: 電気学会論文誌E（センサ・マイクロマシン部門誌） Vol.141 No.7 （2021） 特集：第37回「センサ・マイクロマシンと応用システム」シンポジウム受賞論文

本誌掲載ページ: 215-221 p

原稿種別: 論文／日本語

電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejsmas/141/7/141_215/_article/-char/ja/